一区二区三区无码动态图,精品国产小视频在线观看,www久久无码天堂mv,亚洲第一区二区三区av

歡迎進入上海起發(fā)實驗試劑有限公司!
產(chǎn)品展示

La (SSB) antigen

描述:Identity:La ribonucleoprotein, SSB antigen, Sj?gren syndrome antigen B.Source Material:Bovine thymus (New Zealand origin).Clinical Indications:Autoantibodies present in Sj?gren’s syndrome, systemic lu

更新時間:2016-11-11
訪問次數(shù):2839
廠商性質(zhì):代理商
詳情介紹

La (SSB) ANTIGEN 
AROTEC_La-SSB_Product_Info.pdf Version/Date: B/04.05.20 
ATL01-02 La (SSB) antigen 0.20 mg 
ATL01-05 La (SSB) antigen 0.50 mg 
ATL01-10 1.0 mg 
_________________________________________________________________________________
Description of the Product
Purified from bovine thymus. After coating onto ELISA plates 
the product will bind autoantibodies to La (SSB). 
Purity: The La autoantigen (45-50 kDa) is more than 90% 
pure, as assessed by SDS polyacrylamide gel electrophoresis. 
Concentration: 0.1-1.0 mg protein/ml. 
Storage: The product is stabilised with 20% glycerol and 0.1% 
Micr-O-protectTM. Store at -20 o
C or below (long term) or at 
+4o
C (short term). Avoid repeated freezing and thawing. Mix 
thoroughly before use. 
Clinical and Biochemical Data 
Sjögren's syndrome (SS) is a common systemic autoimmune 
inflammatory disorder characterised by lymphocyte-mediated 
destruction of exocrine glands leading to diminished or absent 
glandular secretion1-4. SS may present as a primary disease 
or in association with other systemic autoimmune diseases 
(referred to as secondary SS). Autoantibodies to the La (SSB) 
antigen can be detected in the sera of up to 87% of patients 
with primary or secondary SS5,6. The presence of anti-La 
(SSB) autoantibodies usually coincides with the presence of 
anti-Ro (SSA) autoantibodies7
, however the fact that anti-Ro 
autoantibodies are far more common in other rheumatological 
conditions such as systemic lupus erythematosis (SLE) and 
mixed connective tissue disease (MCTD) suggests that anti-La 
is more specific for primary and secondary SS than anti-Ro8,9

Anti-La autoantibodies have also been reported to be present 
in other clinical conditions, most notably in the sera of mothers 
of infants with neonatal lupus syndrome10, but also in 10 to 
15% of SLE patients11,12

binds to the oligo(U) 3' termini of nascent 
RNA polymerase III transcripts and facilitates transcriptional 
termination and reinitiation by this enzyme13,-17. It has also 
been reported to function as an ATP-dependent helicase able 
to melt RNA-DNA hybrids18. La (SSB) may be involved in 
other processes as well such as maturation and/or nuclear 
export of RNA polymerase III products and some aspects of 
translation19,20. La (SSB) is a highly phosphorylated protein 
which migrates at about 50 kDa in SDS-polyacrylamide gel 
electrophoresis21. Phosphorylated residues are present at the 
carboxy-terminal part of the protein22. At least 8 isoelectric 
forms (pI range 6 to 7) have been identified23

The amino acid sequences of both human and bovine La 
(SSB) antigen have been determined by cDNA cloning and 
sequencing19,28. Comparison of the two sequences shows 22 
largely conservative amino acid substitutions with a total of 
95% identity. Three regions of the La molecule (amino acids 
1-107, 111-242 and 346-408) are thought to contain the major 
epitopes reactive with human anti-La sera19,24. The broad 
cross-reactivity of patient sera with La (SSB) from diverse 
mammalian species indicates the presence of conserved 
epitopes25. The use of bovine for the 
detection of human anti-La (SSB) antibodies has been 
described by several authors25-27
.
Methodology
The following is an ELISA procedure which can be used to 
detect anti-La (SSB) autoantibodies in human serum using the 
ATL01 purified autoantigen: 
1. Dilute the purified antigen to 0.5-1.0 µg/ml in PBS (10 mM 
potassium phosphate, pH 7.4, 0.15 M NaCl). 
2. Coat ELISA plates with 100 µl of diluted antigen per well. 
Cover and incubate 24 hours at +4o
C. 
3. Empty the plates and remove excess liquid by tapping on a 
paper towel. 
4. Block excess protein binding sites by adding 200 µl PBS 
containing 1% BSA per well. Cover and incubate at +4o

overnight. 
5. Empty plates and apply 100 µl of serum samples diluted 
1:100 in PBS / 1% BSA / 1% casein / 0.1% Tween?
 20. 
Incubate at room temperature for 1 hour. 
6. Empty plates and add 200 µl PBS / 0.1% Tween?
 20 per 
well. Incubate 5 minutes then empty plates. Repeat this step 
twice. 
7. Apply 100 µl anti-human IgG-enzyme conjugate 
(horseradish peroxidase or alkaline phosphatase) diluted in 
PBS / 1% BSA / 1% casein / 0.1% Tween?
 20 per well and 
incubate for 1 hour. 
8. Repeat step 6. 
9. Add enzyme substrate and stop the reaction when 
appropriate. 
10. Read absorbance in an ELISA spectrophotometer. 
References 
1. Molina, R. et al. (1986) Am. J. Med. 80, 23 
2. Bloch, K.J. et al. (1965) Medicine 44, 187 
3. Fox, R.I. et al. (1986) Arthritis Rheum. 29, 577 
4. Aziz, K.E. et al. (1992) Aust. NZ J. Med. 22, 671 
5. Manoussakis, M.N. et al. (1986) Scan. J. Rheumatol. 61, 89 
6. Harley, J.B. et al. (1986) Arthritis Rheum. 29, 196 
7. Craft, J.E. & Hardin, J.A. (1987) J. Rheumatol. 14 S13, 106 
8. St. Clair, E.W. (1992) Rheum. Dis. Clin. N. America 18, 359 
9. Harley, J.B. (1989) J. Autoimmun. 2, 383 
10. Buyon, J.P. et al. (1989) J. Clin. Invest. 84, 627 
11. Reichlin, M. (1986) J. Clin. Immunol. 6, 339 
12. Wiascewk, C.A. & Reichlin, M. (1982) J. Clin. Invest. 69, 835 
13. Stefano, J.E. (1984) Cell 36, 145 
14. Gottlieb, E. & Steitz, J.A. (1989) EMBO J. 8, 841 
15. Gottlieb, E. & Steitz, J.A. (1989) EMBO J. 8, 851 
16. Maraia, R.J. et al. (1994) Mol. Cell Biol. 14, 2147 
17. Maraia, R.J. (1996) Proc. Natl. Acad. Sci. USA 93, 3383 
18. Bachmann, M. et al. (1990) Cell 60, 85 
19. Chambers, J.C. et al. (1988) J. Biol. Chem. 263, 18043 
20. Bachmann, M. et al. (1989) Mol. Cell Biochem. 85, 103 
21. Pruijn, G.J.M. (1994) Man. Biol. Markers Dis. (Kluwer Acad. 
 Publ.) B4.2/1-14 
22. Pfeifle, J. et al. (1987) Biochim. Biophys. Acta 928, 217 
23. Francoeur, A.M. et al. (1985) mol. Cell Biol. 5, 586 
24. McNeilage, L.J. (1990) J. Immunol. 145, 3829 
25. Chan, E.K.L. & Tan, E.M. (1987) J. Exp. Med. 166, 1627 
26. Zhang, W. & Reichlin, M. (1996) Arthritis Rheum. 39, 522 
27. Chan, E.K.L. et al. (1986) J. Immunol. 136, 3744 
28. Chan, E.K.L. et al. (1989) Nucleic Acids Res. 17, 2233 
Micr-O-protect is from Roche Diagnostics GmbH (Mannheim, 
Germany). 
Tween?
 20 is a registered trademark of ICI Americas Inc. 
NOTE: No patented technology has been used by AroTec 
during the preparation of this product. 

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
上一條:Jo-1 antigen
下一條:Nucleosome antigen
人妻中出受孕 中文字幕在线| 亚洲欧美另类精品在线观看| 国产一区二区精品一区二区| 中国产无码一区二区三区| 国产呦系列一区二区三区| 麻豆国产尤物av尤物在线观看| 国产成人亚洲精品无码H| 久热中文字幕在线精品观| 国产成人a视频在线观看| 欧美国产精品 一区二区| 日韩欧美国产精品亚洲二区| 国产911视频在线观看| 99热这里只有精品98| 亚洲老年人操逼一级网站| 国产精品久久久久久精品毛片| 欧美区 亚洲区 国产区| 欧美精品videosex性欧美| 久久久亚洲精品| 一区二区三区 日韩精品| 欧美日韩视频在线第一区| 大香蕉久久精品在线观看| 久久www免费人成一看片| 日韩精品成人一区二区在线| 综合久久997| 欧美性生交18xxxxx无码| 免费无码aⅤ视频免费看| 中文国产人精品久久蜜桃| 久久久久久久久久久久久| 乱人伦人妻精品一区二区| 中文字幕亚洲制服在线看| 久久久免费一区二区三区| 大粗鳮巴久久久久久久久| 亚洲精品久久久久av无码| 国产真实乱在线观看免费| 久久精品 91大神 一区| 午夜亚洲色图福利视频合集| 伊人网在线综合一区二区| 五月天精品视频在线观看| 青青操在线免费手机视频| 亚洲AV片劲爆在线观看| 全部精品孕妇色视频在线|